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lentiviral vectors plko.1 encoding scrambled shctrl  (Addgene inc)


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    Structured Review

    Addgene inc lentiviral vectors plko.1 encoding scrambled shctrl
    H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation <t>of</t> <t>PHLDA1.</t> ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control <t>(shctrl)</t> or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.
    Lentiviral Vectors Plko.1 Encoding Scrambled Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral vectors plko.1 encoding scrambled shctrl/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    lentiviral vectors plko.1 encoding scrambled shctrl - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells"

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells

    Journal: Journal of Cancer

    doi: 10.7150/jca.45262

    H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.
    Figure Legend Snippet: H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.

    Techniques Used: Expressing, Control, Western Blot, Marker

    ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1 . Apoptosis assay of 2008 ( A ) and SKOV3 ( B ) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). ** P <0.001 compared with the shctrl group. Mean ± SD, n = 3.
    Figure Legend Snippet: ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1 . Apoptosis assay of 2008 ( A ) and SKOV3 ( B ) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). ** P <0.001 compared with the shctrl group. Mean ± SD, n = 3.

    Techniques Used: Apoptosis Assay, Expressing

    Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. ( A ) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H 2 O 2 . ( B and C ) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 ( B ) and SKOV3 ( C ) cells after treatment with 0.2 mM H 2 O 2 .
    Figure Legend Snippet: Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. ( A ) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H 2 O 2 . ( B and C ) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 ( B ) and SKOV3 ( C ) cells after treatment with 0.2 mM H 2 O 2 .

    Techniques Used: Expressing, Western Blot



    Similar Products

    lentiviral vectors plko.1 encoding scrambled shctrl  (Addgene inc)
    90
    Addgene inc lentiviral vectors plko.1 encoding scrambled shctrl
    H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation <t>of</t> <t>PHLDA1.</t> ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control <t>(shctrl)</t> or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.
    Lentiviral Vectors Plko.1 Encoding Scrambled Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral vectors plko.1 encoding scrambled shctrl/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    lentiviral vectors plko.1 encoding scrambled shctrl - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    encoding scrambled shctrl  (Addgene inc)
    93
    Addgene inc encoding scrambled shctrl
    Figure 2. H2O2-induced apoptosis of ovarian cancer cells after downregulation <t>of</t> <t>PHLDA1.</t> (A and B) Flow cytometric analysis of 2008 cells (A) and SKOV3 cells (B) expressing control <t>(shctrl)</t> or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, *P<0.05, **P<0.001, compared with the shctrl group. (C and D) Western blot analysis of the apoptosis marker c-PARP1 in 2008 (C) and SKOV3 (D) cell lines expressing shctrl or shPHLDA1.
    Encoding Scrambled Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/encoding scrambled shctrl/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    encoding scrambled shctrl - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: H 2 O 2 -induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. ( A and B ) Flow cytometric analysis of 2008 cells ( A ) and SKOV3 cells ( B ) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, * P <0.05, ** P <0.001, compared with the shctrl group. ( C and D ) Western blot analysis of the apoptosis marker c-PARP1 in 2008 ( C ) and SKOV3 ( D ) cell lines expressing shctrl or shPHLDA1.

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT CCT ACT CGA GTA GGA ATT ACT ACG GAT TAG GTT TTTG), shPHLDA1-2 (CCG GCA GAT CAA GTA GTT TGG ACA TCT CGA GAT GTC CAA ACT ACT TGA TCT GTT TTT TG), and shPHLDA1-3 (CCG GCG AGC ACA TTT CTA TTG TCT TCT CGA GAA GAC AAT AGA AAT GTG CTC GTT TTT TG).

    Techniques: Expressing, Control, Western Blot, Marker

    ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1 . Apoptosis assay of 2008 ( A ) and SKOV3 ( B ) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). ** P <0.001 compared with the shctrl group. Mean ± SD, n = 3.

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1 . Apoptosis assay of 2008 ( A ) and SKOV3 ( B ) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). ** P <0.001 compared with the shctrl group. Mean ± SD, n = 3.

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT CCT ACT CGA GTA GGA ATT ACT ACG GAT TAG GTT TTTG), shPHLDA1-2 (CCG GCA GAT CAA GTA GTT TGG ACA TCT CGA GAT GTC CAA ACT ACT TGA TCT GTT TTT TG), and shPHLDA1-3 (CCG GCG AGC ACA TTT CTA TTG TCT TCT CGA GAA GAC AAT AGA AAT GTG CTC GTT TTT TG).

    Techniques: Apoptosis Assay, Expressing

    Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. ( A ) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H 2 O 2 . ( B and C ) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 ( B ) and SKOV3 ( C ) cells after treatment with 0.2 mM H 2 O 2 .

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. ( A ) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H 2 O 2 . ( B and C ) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 ( B ) and SKOV3 ( C ) cells after treatment with 0.2 mM H 2 O 2 .

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT CCT ACT CGA GTA GGA ATT ACT ACG GAT TAG GTT TTTG), shPHLDA1-2 (CCG GCA GAT CAA GTA GTT TGG ACA TCT CGA GAT GTC CAA ACT ACT TGA TCT GTT TTT TG), and shPHLDA1-3 (CCG GCG AGC ACA TTT CTA TTG TCT TCT CGA GAA GAC AAT AGA AAT GTG CTC GTT TTT TG).

    Techniques: Expressing, Western Blot

    Figure 2. H2O2-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. (A and B) Flow cytometric analysis of 2008 cells (A) and SKOV3 cells (B) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, *P<0.05, **P<0.001, compared with the shctrl group. (C and D) Western blot analysis of the apoptosis marker c-PARP1 in 2008 (C) and SKOV3 (D) cell lines expressing shctrl or shPHLDA1.

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells.

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: Figure 2. H2O2-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. (A and B) Flow cytometric analysis of 2008 cells (A) and SKOV3 cells (B) expressing control (shctrl) or PHLDA1-targeting shRNAs (shPHLDA1). Lower right and upper right quadrants showed early apoptotic and late apoptotic/necrotic cells, respectively. n=3, *P<0.05, **P<0.001, compared with the shctrl group. (C and D) Western blot analysis of the apoptosis marker c-PARP1 in 2008 (C) and SKOV3 (D) cell lines expressing shctrl or shPHLDA1.

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT

    Techniques: Expressing, Control, Western Blot, Marker

    Figure 3. ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. Apoptosis assay of 2008 (A) and SKOV3 (B) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). **P<0.001 compared with the shctrl group. Mean ± SD, n = 3.

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells.

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: Figure 3. ER stress-induced apoptosis of ovarian cancer cells after downregulation of PHLDA1. Apoptosis assay of 2008 (A) and SKOV3 (B) cells expressing shctrl or shPHLDA1 after treatment with thapsigargin (Tg). **P<0.001 compared with the shctrl group. Mean ± SD, n = 3.

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT

    Techniques: Apoptosis Assay, Expressing

    Figure 5. Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. (A) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H2O2. (B and C) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 (B) and SKOV3 (C) cells after treatment with 0.2 mM H2O2.

    Journal: Journal of Cancer

    Article Title: PHLDA1 Modulates the Endoplasmic Reticulum Stress Response and is required for Resistance to Oxidative Stress-induced Cell Death in Human Ovarian Cancer Cells.

    doi: 10.7150/jca.45262

    Figure Lengend Snippet: Figure 5. Expression of autophagy-related proteins, anti-apoptosis proteins, ER stress-associated proteins after PHLDA1-downregulation in ovarian cancer cells. (A) Western blot analysis of Autophagy-related proteins (Beclin-1, P62, and LC3) and anti-apoptosis protein (Bcl-2) in 2008 cells expressing shctrl or shPHLDA1 after treatment with 0.2 mM H2O2. (B and C) Western blot analysis of ER stress-associated proteins (IRE1α, PERK, BIP, ERO1-Lα, and PDI) in 2008 (B) and SKOV3 (C) cells after treatment with 0.2 mM H2O2.

    Article Snippet: Cells were infected by lentiviral vectors pLKO.1 encoding scrambled shctrl (Addgene, Cambridge, MA, USA) or one of the three PHLDA1-targeting shRNAs (all from Sigma, St. Louis, MO, USA): shPHLDA1-1 (CCG GCC TAA TCC GTA GTA ATT

    Techniques: Expressing, Western Blot